Effect of sevoflurane preconditioning combined with postconditioning on ET-1 and TNF-αexpression in rats during lung ischemia-reperfusion
DU Ning
XU Gui-ping
LIU Bei
Abstract：Objective To investigate the effects of sevoflurane preconditioning combined with postconditioning on ex-pression of plasma ET-1 and lung tissue TNF-αin rats during lung ischemia-reperfusion.Methods Fifty-four SD rats were randomly divided into three groups, which were sham group ( S group), lung ischemia-reperfusion group ( I/R group), sevoflurane pretreatment and post-treatment combination group (Spr+o group), with 18 rats in each group.Rats in I/R group, Spr+o group were made I/R models by using the modified Epinger lung ischemia-reperfusion method.Rats in S group was only isolated pulmonary hilum but not ligated.Rats in Spr+o group were inhaled 2.1%sevoflurane for 30 minutes before ischemia, then the left pulmonary hilum were clamped for 45 minutes and were inhaled 2.1%sevoflurane for 30 minutes fol-lowed a 90 minutes reperfusion.S group and I/R group were not given sevoflurane intervention.At 30, 60 and 120 minutes reperfusion, six rats in each group were randomly selected to sacrifice in order to measure the lung tissue wet/dry ( W/D) weight ratio.ELISA assay was used to test the plasma ET-1 and lung tissue TNF-αlevels.Pathological changes in the lung tissue were observed by using HE staining.Results Compared with the S group, lung tissue W/D, TNF-αand plasma ET-1 levels of Spr+o group and I/R group were increased at each time point reperfusion (P<0.05).Compared with the I/R group, lung tissue W/D, TNF-αand plasma ET-1 levels of Spr+o group were decreased at each time point reperfusion ( P<0.05).Microscopic examination showed that the pathologic changes were significantly attenuated in Spr+o group compared with I/R group.Conclusions Sevoflurane preconditioning combined with postconditioning can reduce lung ischemia-reperfu-sion injury, which may be related to the inhibition of plasma ET-1 and lung TNF-αexpression.
Keywords：sevofluranepretreatmentafter treatmentreperfusion injurylungendothelin-1tumor necrosis factor-α
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-3,7 )