Effect and mechanism of PPAR-γon cisplatin resistance reversal of human nasopharyngeal carcinoma CNE1/DDP cells
LEI Chen
ZHENG Shu-yan
HUANG Yong-wang
Abstract：Objective To investigate the effect of PPAR-γon cisplatin sensitivity reversal of human nasopharyngeal carcinoma CNE1/DDP cell line and its mechanism.Methods The human nasopharyngeal carcinoma CNE1/DDP cells were treated by 10 μg/mL and 20 μg/mL PPAR-γagonist rosiglitazone.MTS assay was used to measure the effect of pro-liferation;flow cytometry was used to measure the cell apoptosis and P-gp expression;RT-PCR and Western blot assay were used to measure the mRNA and protein expression of multidrug resistance gene (MDR1), multidrug resistance-associated protein ( MRP) , B-cell lymphoma gene 2 ( bcl-2);Western blot assay was used to measure the phosphorylation of Akt ( p-Akt).Results CNE1/DDP cells were treated by 10μg/mL and 20μg/mL rosiglitazone, the drug resistance reverse rates were 100.0%, 149.3%±11.3%, 293.8%±25.5%, respectively;the apoptosis rate was gradually increased;the mR-NA and protein expression of PPAR-γ, MDR1, MRP and bcl-2, and p-Akt expression were gradually decreased (all P<0.05).Conclusions PPAR-γcould increase cisplatin sensitivity of CNE1/DDP cells by down-regulating phosphorylation of Akt and the expression of MDR1 and MRP.
Keywords：nasopharyngeal carcinomaPPAR-γCNE1/DDP cellcisplatindrug resistance geneapoptosis
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 10-12,15 )