Changes of immune related gene-expression profiling in rats with sepsis
MAO Ying-ying
WANG Dong-qiang
LI Zhi-jun
Abstract：Objective To observe the change of immune related gene-expression profiling in rats with sepsis.Meth-ods 120 male wistar rats were randomly divided into sham operation group ( n=30 ) and model group ( n=90 ) .The model group according to different time points (24 h, 48 h and 72 h) were randomly divided into 3 groups ( each subgroup n=30).The rats of model group received cecal ligature and puncture (CLP) operation.Gene chips containing 28 000 rot-genes cDNA were used to test gene-expression in liver of septic rats to select the genes with different expressions with soft-ware.Results Model group had 671 differential expression genes with 97 immune related genes at 24 h;133 differential expression genes with 36 immune related genes at 48 h;204 differential expression genes with 33 immune related genes at 72 h.Three time points in model group and sham operation group repeated gene were 58, immune related gene 15.Con-clusion A series of cell defense and immune function genes change significantly in liver tissue of septic rats, which may be involved in the pathophysiology of sepsis induced hepatic injury in different extent.
Keywords：sepsisgene chipgene expressioncecal ligation and perforationrat
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 4-6,9 )