Effect of hypoxia-mimic deferoxamine on fibroblast growth factor 21 expression in intestine cells
HE Xue-qian
ZHANG Yi
WU Ling-jian
CHEN Chao
XIE Xiao-qi
CHEN LI Bin-ji
LIU Yan-long
LIN Hong
PANG Ling-xia
Abstract：Objective To study the effect of hypoxia-mimic deferoxamine ( DFO ) on fibroblast growth factor (FGF21) expression in intestinal Caco-2 cells.Methods Caco-2 cells were either treated with 50, 100, 150, 200μmol/L DFO for 12 h or treated with 200 μmol/L DFO for 4, 8, 12 h.FGF21 mRNA expression were measured by RT-PCR. Caco-2 cells were pretreated with either HIF or ROS inhibitor and then treated with DFO.FGF21 mRNA expression was measured by RT-PCR.Results FGF21 mRNA expression decreased in dose-and time-dependent manners with DFO treat-ment in Caco-2 cells.HIF inhibitor and DFO treatment still decreased FGF21 mRNA expression (all P<0.05).But ROS inhibitor pretreatment reversed DFO-induced FGF21 mRNA down-regulation (all P>0.05).Conclusion DFO-induced FGF21 down-regulation is not associated with HIF stabilization, but involves hypoxia-induced oxidative stress.
Keywords：hypoxiadeferoxamineCaco-2 cellsfibroblast growth factor
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1-3 )