Effects of Lovastatin on the proliferation and activity of iNOS-NO system in rat cardiac fibroblasts induced by aldosterone
FENG Hui-ping
JIA Xin-wei
WANG Yan-fei
ZHANG Fang
ZHANG Lan-fang
XIE Jun-min
Abstract：Objective To investigate the effects of Lovastatin on the aldosterone-induced proliferation of rat cardiac fi-broblasts(CFs) and the activity of inducible nitric oxide synthase (iNOS).Methods CFs were isolated from neonatal Sprague Dawley rats by trypsin digestion.MTT colorimetric assay was used to evaluate cell proliferation.Nitric acid reduc-tase method, spectrophotometry and reverse transcription-polymerase chain reaction were used to detect the NO contents, iNOS activity and iNOS mRNA expression respectively.Results Lovastatin(1 ×10 -8 -1 ×10 -5 mol/L) inhibited the proliferation of CFs increased NO contents and iNOS activity in a dose-depend manner(P<0.05), which could be reversed by mevalonate(1 ×10 -3 mol/L) or farnesyl pyrophosphate(5μmol/L).NO contents and iNOS activity, iNOS activity and iNOS mRNA expression were positively correlate induced by different concentrations of Lovastatin(r=0.826, 0.752, re-spectively, all P<0.01).The proliferation of CFs was significant negatively correlated with NO contents induced by differ-ent concentrations of Lovastatin(r=-0.908, P<0.01).Conclusions The results indicate that Lovastatin up-regulated iNOS-NO system in CFs, and inhibite proliferation of CFs in a dose-depenent manner, which can be related to the regula-tion of mevalonate pathway.
Keywords：Lovastatinaldosteronenitric oxidenitric oxide synthasecardiac fibroblasts
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 8-11 )