Expression of Hes1, cell count, cell proliferation of bone marrow CD3+4 cells in acute T-ALL patients
TIAN Chen
WANG Chao-yu
JIA Yong-sheng
ZHANG Yi-zhuo
Abstract：Objective To study the expression of Hes1, cell cycle, cell proliferation of bone marrow CD3+4 cells in acute T lymphoblastic leukemia( T-ALL) patients.Methods Samples of 8 T-ALL patients and 4 healthy donars were collected. Hes1 expression was analyzed by real time PCR.Bone marrow mononuclear cells were isolated with Ficoll and then the pro-portion and cell cycle of CD3+4 cells were analyzed by FACS, CD3+4 cells were cultured in vitro for colony formation cells ( CFC) .After increasing the expression of Hes1 in CD3+4 cells, cell cycle was analyzed by FACS and the colony formation of CD3+4 cells were analyzed by CFC.Results Hes1 expression in T-ALL and control were 3.3 ±0.8 and 1 respectively(P<0.05).The ratio of CD3+4 cells in T-ALL and control were 0.02%±0.003%and 0.06%±0.005%respectively(P<0.05). The proportion of S phase of CD3+4 cells in T-ALL and control were 16.2%±0.98%and 28.0%±1.12%respectively(P<0.05).The proportion of G0 phase of CD3+4 cells were 19.0%±0.9%and 9.0%±0.5%respectively(P<0.05).The colo-ny formation of CD3+4 cells from patients was lower than that from normal donors.More CD3+4 cells went into quiescence and the proliferation of these cells were inhibited after overexpressing Hes1.Conclusions In T-ALL patients, the proportion of CD3+4 cells in bone marrow is lower.More of these cells go into quiescence which is related to Hes1.
Keywords：acute T lymphoblastic leukemiaCD3+4 cellHes1 genecell cycleproliferation
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 5-7 )