Effects of 1,25(OH)2D3 on proliferation and differentiation of osteoblasts and influence on the adhesion of breast cancer cells and the osteoblasts

WU Yu; WEI Hong-yan; ZHAI Qiong-Li

doi:10.3969/j.issn.1002-266X.2014.39.001

Abstract：Objective To investegate the effect of 1,25(OH)2D3 on osteoblastic proliferation and differentiation and to detecte the influence of 1,25(OH)2D3 on the adhesion of breast cancer cells and the osteoblasts.Methods The human osteolasts(hFOB1.19) were divided into conrol group, ethanol group and 1,25(OH)2D3 groups which were cultured and treated in normal DMEM/F12 medium, DMEM/F12 medium with 0.1 ml/L ethanol and DMEM/F12 medium with different concentrations of 1,25(OH)2D3 respectively.The cellular proliferation inhibition rate of human osteoblasts was measured by CCK-8 assay.The ALP activity was measured by trace enzyme assay.The adhesion of human breast cancer cell lines and hFOB1.19 were detected by cell adhesion method.Results The proliferation inhibition rate of osteoblasts cultured in control group, ethanol group or 1,25(OH)2D3 groups whoes concentration was 1 ×10 -7, 1 ×10 -8, 1 ×10 -9, 1 ×10 -10 mol/L were 0, -1.63%±2.04%, 16.02%±1.13%, 10.78%±0.98%, 8.82%±2.59%, 5.55%±0.57%respec-tively(P<0.05) at the third day.The ALP activity in each group was (1.77 ±0.03), (1.76 ±0.01), (2.11 ±0.02), (1.96 ±0.01), (1.84 ±0.04), (1.81 ±0.03) King unit/100 mL respectively.The adhesion of MDA-MB-231 and hFOB1.19 cells cultured in each group was 58.80%±3.11%, 59.80%±3.96%, 42.00%±1.87%, 46.40%± 2.30%, 53.80%±0.84%, 57.00%±3.24% respectively.The adhesion of T47D and hFOB1.19 cells cultured was 55.75%±2.22%, 56.25%±1.71%, 47.80%±3.11%, 50.60%±2.07%, 54.40%±2.70%, 57.20%±1.48%respectively.There were statistically significant in the ALP activity, adhesion of MDA-MB-231 and hFOB1.19 cells cul-tured, adhesion of T47D and hFOB1.19 cells cultured among 1,25(OH)2D3 groups whoes concentration was 1 ×10 -7, 1 ×10 -8 with other groups.Conclusions 1,25(OH)2D3 can inhibit the proliferation of osteoblasts and promote the activi-ty of ALP.It can also inhibit the cell adhesion of breast cancer cells and osteoblast cells.