Effects of short-term caloric restriction on insulin sensitivity in normal rats
PIAO Xiong
LU Huan-jun
LI Xiang-Lan
JIANG Hai-ying
Abstract：Objective To investigate the effect of the short-term caloric restriction( CR) on insulin sensitivity and its signal transduction mechanisms.Methods A total of 24 F344/NSIc rats were equally divided into ad libitum group ( AL group) and CR group.Rats in AL group were free fed every alternate day for 8 weeks, while rats in CR group were fed re-strict caloric without water deprivation for 8 weeks.Glucose infusion rate(GIR) and insulin sensitivity were measured by u-sing hyperinsulinemic-euglycemic clamp method.The body weight mass and visceral fat weight between the two groups were compared.The muscle glucose transporter 4(GluT4), kinase substrate 160(AS160 ) protein and p-AS160 protein was de-tected by using Western blot method.Results GIR in CR group was obviously higher than that of control group, while body mass and visceral fat weight significantly lower than control group.However, there was no significant difference in ex-pression of GluT4, AS160 protein and p-AS160 protein.Conclusions Short-term caloric restriction can improve the insu-lin sensitivity in healthy rats which may be associated with reduced the visceral fat increasing.
Keywords：caloric restrictioninsulin sensitivityglucose transporter 4kinase substrate 160
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 29-31 )