Treatment effect of bone marrow mesenchymal stem cells transplantation on the severe acute pancreatitis in rats
WANG Zhong-qiong
LIU Yi
XIA Guo-dong
YANG Chun
ZHONG Xiao-lin
Abstract：Objective To study the protective effect and the possible mechanisms of transplantation bone marrow mes-enchymal stem cells (MSCs) on pancreatic injury in rats with severe acute pancreatitis(SAP).Methods A total of 120 male Sprague-Dawley rats were randomly divided into sham operation group( N group) , severe acute pancreatitis group ( P group) , transplantation MSCs group( M group) .The dose of 5%sodium taurocholate was retrograde injected into the pan-creatic duct of the rats in P group and M group to induce SAP model.Rats in N group whose pancreases were just flipped and striked gently without injection.MSCs were transplanted into rats in M group via portal vein, while the same dosage of 0.9%NS was injected by the same vein routine in P group.Ten rats in each group were sacrificed at 24 h, 48 h, 72 h lat-er.Pancreas and kidney damage of each group were observed under gross morphology and optical lens.The serum AMY, BUN, Cr, TNF-αand IL-6 level of each group were tested.Results Pancreas and kidney injury in P group were signifi-cantly heavier.The content of serum AMY, BUN, Cr, TNF-αand IL-6 level in P group were both higher than those of N group(P<0.05).Pancreatic and renal tissue injury in M group rats mitigated.The content of serum AMY, BUN, Cr, TNF-αand IL-6 level in M group were both lower than those of P group (P<0.05).Conclusions Transplantation via portal vein MSCs is an effective treatment on SAP, which can reduce the occurrence of multiple organ dysfunction and im-prove prognosis.
Keywords：bone marrow mesenchymal stem cellscell transplantationsevere acute pancreatitis
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 26-28 )