Protection of TEMPO for liver function of cirrhotic rat after hepatic porta blocking

SU Yong-hui; XU Jia-qi; BU Ju-yuan; LI Xiao-ling; HOU Bing-zong

doi:10.3969/j.issn.1002-266X.2014.38.08

Abstract：Objective To investigate the protection of 4-Hydroxy-2.2.6.6-etram-ethyl-piperidinooxy(TEMPO) for liver function of cirrhotic rat after hepatic porta blocking.Methods The traditional carbon tetrachloride ( CCl4 ) method was used to induce liver cirrhosis in 90 adult rats and then equally divided into three groups, which were control group, model group and treatment group.Rats in model group and treatment group were adopted by open hepatic door and interdict operation to block liver door for 30 minutes and then restore blood flow ( rats in control group were adopted by exposed porta only without block) .Preoperative model group and control group were used normal saline ( as the volume of treatment group use) to intravenous injection slowly before operation and every 12 h after operation.Rats in treatment group were intrave-nous injected TEMPO at 10 mg/100 g (10 times diluted with saline water) before operation and static note once every 12 h after operation.All rats were taking the liver tissue and blood samples after operation 72 hours later.The liver tissue patho-logical changes and cell apoptosis were observed.The level of superoxide dismutase( SOD) , peroxidase ( CAT) ,glutathione peroxidase ( GSH-Px) activity and malondialdehyde ( MDA) of liver tissue clear liquid were tested.The liver function of three groups were tested.Results The SOD, CAT and GSH-Px activity of liver tissue in treatment group significantly higher than the model group(P<0.05).The MDA level in treatment group was significantly lower than model group(P<0.05), while liver function index was significantly lower than model group(P<0.05).Conclusions TEMPO can protect the rat liver function of cirrhotic rat after hepatic porta blocking.