Expression and significance of macrophage migration inhibition factor and iNOS in fetal membranes of pregnant women with premature rupture of fetal membranes
XIE Xiao-fang
WANG Wei-zhen
Abstract：Objective To study the effect of macrophage migration inhibition factor( MIF) and inducible nitric oxide synthase (iNOS) in the development of premature rupture of fetal membranes(PROM).Methods A total of 90 parturient women who had delivered by cesarean section before labor were selected in the study and were divided into 3 groups, which were preterm premature rupture of the membranes group ( pPROM group, 30 cases) , term premature rupture of the mem-branes group(tPROM group, 30 cases) and full-term cesarean section delivery group (control group, 30 cases).The membranes were collected from the >2 cm point of rupture of membranes about 1 cm ×1 cm in cesarean section after preg-nancy termination with placenta deliveried and made into paraffin sections.Chorioamnionitis was identified by using HE staining to determine the neutrophilic granulocyte infiltration.The distributions and expression levels of MIF and iNOS in fetal membranes were detected by immuno histochemical method.The expression of MIF and iNOS in the three groups were analyzed.Results A total of 27 cases chorioamnionitises were found.MIF and iNOS located in cytoplasm of amnio-endo-thelial cells.The expression of MIF in fetal membrane cells in pPROM group and tPROM group were higher than that in control group(P<0.01).The expression of iNOS in pPROM group was higher than that in control group (P<0.01).The expression levels of MIF and iNOS in chorioamnionitis puerperas were significantly higher than those in non-chorioamnionitis puerperas(P<0.05).Conclusions The expression of MIF and iNOS increase in fetal membrane of RPOM puerperas. The overexpression of MIF and iNOS may promote the development of PROM and chorioamnionitis.
Keywords：macrophage migration inhibition factorinducible nitric oxide synthasepremature rupture of fetal mem-branechorioamnionitis
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 11-14 )