Effect of domestic decitabine influence on morphology, proliferation and CD80 expression in mouse lymphoma/leukemia cell line EL4 cells
WANG Li-Xin
DING Jie
CHEN Xiao-Ping
HU Xiao-Shan
YAO Yu-Shi
Abstract：Objective To compare the influence of cytotoxicity and CD80 expression in EL4 cells, following in vitro treatment with decitabine ( DEC) manufactured internal.Methods EL4 cell line was treated with Dacogen ( DAC, manu-factured by Xian Janssen Pharmaceutical Ltd) or DEC (final concentration 0.25μmol/L) in vitro for 3 days.Morphology was analysised by method of Wright-Gimesa staining.CD80 expression was detected by method of flow cytometry.Cell pro-liferation was detected by method of CCK-8 kit.Results DAC and DEC treatment resulted in similar morphological chan-ges in EL4 cells.Moreover, effects of CD80 expression and inhibited proliferation induced by both DAC and DEC in EL4 cells were no significant differences (P>0.05).Conclusions There was no significant difference between DAC and DEC in the ability of change cellular morphology, inhibit proliferation and stimulate CD80 expression in mouse-lymphoma/leuke-mia cell EL4 cultured in vitro, which indicates the effects of the two drugs were similarity in both cytotoxic effect on tumor cells and stimulation of anti-tumor immune response when administered in vivo.
Keywords：decitabinelymphoma EL4 cellsproliferationinhibitioncostimulatory molecules
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 5-7 )