MicroRNA expression profiles of hepatic tissue in primary hepatic carcinoma patients with different blood stasis syndrome
LI Meng-ping
CAO Hai-ming
WU Zhe-li
YE Xiao-wei
CHEN Xin-lin
FU Shun-jun
TAO Wen-hui
Abstract：Objective To provide an objective quantitative standard for traditional Chinese medicine syndrome-types of primary hepatic carcinoma.Methods The differences about 10 kinds of miRNA expression in hepatic tissue between qi deficiency and blood stasis syndeome, liver stagnation and blood stasis syndeome, gan-dan damp-heat syndeome, liver sta-sis phlegm stagnation syndeome patients with hepatocellular carcinoma were compared by using miRNA microarray and RT PCR method.There were 3 cases in each group which named as their syndrome.All the hepatic tissues were compared with 3 cases of normal liver tissues ( normal group) .Results MiR-122-3p in hepatic tissue was higher than the normal group (P<0.05).MiR-122-3p in qi deficiency and blood stasis syndeome group was higher than the other group(P<0.05). MiR-30b-5p in gan-dan damp-heat syndeome group was lower than the other three groups(P<0.05), which was similar with the normal group.MiR-182-5p in qi deficiency and blood stasis syndeome group, liver stagnation and blood stasis syndeome group, gan-dan damp-heat syndeome group were higher than normal group(P<0.05).MiR-221-5p in liver stag-nation blood stasis syndeome group and gan-dan damp-heat syndeome group were lower than the other groups(P<0.05). But there was no significant difference between the two groups.MiR-221-3p in liver stasis phlegm stagnation syndeome group was higher than the other groups, with no statistical difference.MiR-21-5p in qi deficiency and blood stasis syndeome group, liver stagnation and blood stasis syndeome group were higher than gan-dan damp-heat syndeome group and liver stasis phlegm stagnation syndeome group, but there was no significant difference.There was no significant difference in MiR-222-3p, miR-214-3p, miR-491-3p, miR-422a of each group.Conclusions MiRNA expression in different kinds of blood stasis syndeome in primary hepatic carcinoma patients was different.This research provide an objective quantitative standard for traditional Chinese medicine syndrome differentiation of primary liver cancer classification.
Keywords：primary hepatic carcinomablood stasis syndromesyndrone differentiation dassification Chinese tradi-tional medicine microRNA
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )