Analysis of association between genetic polymorphism of human platelet antigens HPA-1-5 and type 2 diabetes combined with carotid atherosclerosis of Han people in Guiyang
ZHENG Jing
XU Shi-fang
ZHANG Ya-hua
HONG Huan-shan
FAN Liang-min
Abstract：Objective To study the association between genetic polymorphism of human platelet antigens HPA -1-5 and type 2 diabetes combined with carotid atherosclerosis ( CA) of Han people in Guiyang .Methods 99 patients with type 2 diabetes (diabetes group) and 100 healthy controls (normal group) were enrolled in this case-control study.The di-abetes group was devided into two groups , which was CA( +) (combined with CA) and CA( -) (non-CA).Phenol-chloroform method was used to extract the genomic DNA and sequence-specific primers-polymerase chain reaction ( SSP-PCR) technique was applied for amplification of the target gene and genotyping .Results The allele frequency and geno-type of HPA-1, 2, 3, 5 showed significant differences among the normal group , CA(+) group and CA(-) group (all P<0.05).The allele frequency and genotype of HPA-1, 2, 3 showed significant differences between the normal group and the diabetes group (all P<0.05).The allele frequency and genotype of HPA-5 showed significant differences between CA (+) and CA(-) (P<0.05).Logistic regression analysis showed that age , duration of diabetes, hypertension, smok-ing, overweight, dyslipidemia, genetic polymorphism of HPA-5 were risk factors of CA in patients with type 2 diabetes. Conclusions There is a possible correlation between genetic polymorphism of human platelet antigens HPA -1, 2, 3 and occurrence of type 2 diabetes.Genetic polymorphism of HPA-5 may be the risk factor of type 2 diabetes with CA.
Keywords：human platelet antigensgenetic polymorphismtype 2 diabetesatherosclerosis
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 18-21 )