Effects of UPLC analysis conditions on the conversion factor determination of wogonoside
ZHOU Qiong
SHI Jian
WANG Gui-ping
QIAN Fang
ZHANG Yan-tao
XIA Bi-jun
Abstract：Objective To investigate the effects of UPLC analysis conditions on the conversion factor ( K) determina-tion of wogonoside .Methods A series of standard working solutions containing wogonoside and wogonin ( molar ratio 1∶1 ) were prepared and determined by UPLC to obtain peak area ratio of the two compounds , which was equal to the value of K.Then, analysis conditions were altered respectively and observed the variance of K value .Results When pH value of mobile phase A rose from 2.5 to 3.5, 4.5 and 6.5, K values were found to increase 10.7%-14.6%.When the pH value rose to 8.5, the retention time of wogonoside and wogonin moved forward significantly , which affected the accuracy of K value determination .With the variance of ionic strength of mobile phase A and pH value of sample medium , the K values determined were similar (deviation<4%).With the variance of detection wavelength from predetermined 280 nm to 254, 274, 342 nm, deviation of K value was in the range of 4.1%-10.7%.Conclusions The K value determination of wogonoside is susceptible to the variance of several UPLC analysis conditions such as pH value of mobile phase A , detection wavelength .To provide more precise quanification for glucuronides which are usually not available commercially , it is nec-essary to keep UPLC analysis conditions consistent during the process of determination .
Keywords：conversion factorUPLC analysis conditionswogonosidewogonin
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 4-7 )