Effects of sufentanil post-conditioning on Cx43 in myocardial ischemia reperfusion of rats

XU Hong-mei; CHU Hai-chen; NIU Ze-jun; LI Hong-mei

doi:10.3969/j.issn.1002-266X.2014.35.010

Abstract：Objective To investigate the impaction of sufentanil post-conditioning on Cx43 expression of myocardial ischemia reperfusion injury ( I/RI) .Methods Sixty Sprague Dawley( SD) rats were randomly divided into 4 groups ( n=15).The group B:Ligature the left anterior descending coronary artery (LAD) of rat heart for 30 min of ischemia, then 120 min of reperfusion, the myocardial ischemia-reperfusion injury model could be obtained; the group A: only thread, without ligation of LAD for 150 min;the group C:ischemia 30 min line at the end of the 30 s, reperfusion 30 s, repeat 3 times, 120 min of reperfusion;the group D:5 min before 30 min of ischemia reperfusion intravenous drip sufentanil 0.1μg/kg, 120 min of reperfusion.Took out rat heart at the end of reperfusion, then calculated the percentage of infarct size of myocardium ( MIS%) by triphenyltet tetrazolium chloride ( TTC) staining, and observed the content of total connexin 43 (tCx43) and phosphorylated connexin 43 (pCx43) in myocardium by semiquantitative Western blotting.Results The MIS%of group A, B, C, D were respectively 0.8%±2.4%, 44.6%±5.3%, 37.4%±3.7%, 26.5%±4.2%, com-pared with group B and group C, MIS% in the group D was lower significantly, P<0.05.Basing on the electrophoresis banding density of tCx43 and pCx43 of group B, the percentage of density of tCx43 of group A, B, C, D were respectively 2.3, 1.0, 1.3, 1.8;the percentage of density of pCx43 of group A, B, C, D were respectively 2.4, 1.0, 1.3, 1.6. There was little expression of tCx43 and pCx43 in group B;the expression of tCx43 and pCx43 in group D was higher signif-icatively than that in group B and group C (P<0.05).Conclusion Sufentanil may play a protective role on rat myocardi-al ischemia reperfusion injury by increasing the content of Cx43 of rat myocardium after the myocardial ischemia reperfu-sion.