Restorative effect of glial cell line-derived neurotrophic factor by intracerebral infusion on nigrostriatal dopaminergic neurons in rat Parkinson model
XU Jia-jun
YANG Hong-an
WANG Guo-dong
Abstract：Objective To evaluate the restorative effect of glial cell line-derived neurotrophic factor ( GDNF) intrace-rebrallyinfusedonnigrostriataldopaminergicneuronsinratmodelwithParkinsoniandisease.Methods 70ratswereem-ployed for the modeling of Parkinsonian disease with right intracerebral( medial forebrain bundle,MFB; ventral tegmental area,VTA) infusion of 12μg 6-OHDA( 3μg /μL) ,and get 39 rat models.29 rats were received controlled intracerebral human recombined GDNF infusion (intraventricle:n=15, intraputamen:n=14) , while the other 10 rats were given ve-hicle as controls( intraventricle:n=5, intraputamenal: n=5).Immunohistochemistry and quantitative morphological a-nalysis was used to quantify the number and size of tyrosine hydroxylase positive( TH+) dopaminergic neurons and the TH+fiber density in nigrostriatal region.Results In controlled group ,the number and density of TH+dopaminergic neuron-swere in right sids increased than left.(P<0.05).Compared to controls,TH+fiber increased bilaterally in number, size and density in periventricular striatum of lesioned side.Conclusions Intracerebral GDNF infusion induces significant re-storative effect on nigrostriatal dopaminergic neurons.
Keywords：glial cell line-derived neurotrophic factorparkinsonism diseaseimmunohistochemistryquantitative morphology
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 21-23 )