Enhances gemcitabine chemosensitivity and apoptosis of human pancreatic carcinoma cells after decreasing EZH2 level by RNA interference
ZHOU Zhang-ming
CHU Sheng-hua
Abstract：Objective To explore the relationship between the expression of EZH2 and BCNU Chemosensitivity and Apoptosis of human glioma U251 cells by decreasing EZH2 gene expression by RNA interference.Methods One pair of DNA template coding siRNA was synthesized against U251 to reconstruct pRNA-EZH2, which was transfected into U251 cells.The EZH2 expression in U251 cells were transfected with pRNAT-EZH2, cells were exposed to BCNU and it was de-tected by RT-PCR.MTT was used to observe the growth inhibiting ratio induced by BCNU in U251 cells.Cell apoptosis was analyzed by fluorescence microscopy.Caspase-3 activity was measured by spectrofluorometer.Results RT-PCR ana-lyses demonstrated that pRNAT-EZH2 could significantly inhibit the expression of EZH2 in U251 cells (P<0.01); after U251 cells were exposed to BCNU, the growth inhibiting ratio, Caspase-3 activity and cell apoptosis of pRNAT-EZH2 transfected cells was significantly increased compared to that of control group and negative group (P<0.01).Conclusion pRNAT-EZH2 can significantly inhibit the expression of EZH2, increase chemosensitivity to BCNU and apoptosis of U251 cells.
Keywords：E2H2 GENE RNA interferencehuman gliomachemosensitivityapoptosisgemcitabine
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 14-17 )