Relationship of CXCR5-CXCL13 expressing and colorectal carcinoma metastasis together with KRAS mutation

LIU Yan-kui; YOU Qing-jun; JIN Lin-fang; Feng Jing-jie; QI Xiao-wei

doi:10.3969/j.issn.1002-266X.2014.34.001

Abstract：Objective To observe the expression of chemotactic factor CXCR5-CXCL13 in the metastasis lymphono-dus of colorectal carcinoma, and explore the correlation between the expression of CXCR5-CXCL13 and colorectal carcino-ma metastasis and the mutation of KRAS gene.Methods The immunohistochemistry staining method was used to detect the protein expression of CXCR5-CXCL13 in 148 specimens of colorectal cancer and lymphonodus metastasis tissues and to-gether with 95 control samples.The Real-time Polymerase Chain Reaction ( Realtime-PCR) technique was used to examine the mutations of KRAS gene in 148 specimens.We analysis the correlation between expression of CXCR5-CXCL13 and clinical pathological parameters, survival data and KRAS mutation.Results CXCR5 was mainly expressed in epithelial cell while CXCL13 was mostly found in interstitial cell.The protein levels of CXCR5-CXCL13 in colorectal cancer were up-regulated than normal tissues (P<0.05), and also higher in metastasis lymphaden than normal ones (P<0.05).The protein levels of CXCR5 and CXCL13 in colorectal cancer and metastasis lymphaden correlated positively ( P<0.05 ) . Conclusions CXCR5-CXCL13 may play an important role in development, metastasis and relapse of colon cancer, and which can be used as a prognostic marker of colorectal carcinoma.CXCR5-CXCL13 expressing and KRAS mutation turn up at the same time, which may imply they interactive with each other.The research of CXCR5-CXCL13 may offer a new drug-target for the anti-colorectal carcinoma.