Formation mechanism of radiation fibrosis after introoperative radiotherapy in rectum tissue of rabbits with tumor of pelvis

CHEN Hai-yan; YANG Xu; WANG Pei-liang

doi:10.3969/j.issn.1002-266X.2014.33.003

Abstract：Objective To discuss formation mechanism of radiation fibrosis after introoperative radiotherapy in rabbits with tumor of pelvis .Methods 20 big female New Zealand albino rabbits were randomly allocated into control group , irra-diation of 4 weeks group , irradiation of 12 weeks group , and irradiation of 16 weeks group .Except control group , other groups all received irradiation .Pathological changes in rectum tissues were observed by light microscope .Proteins of rec-tum were extracted , and expressions of TGF-βor NF-κB were detected by Western blot .Results In rectum tissue sections of control group , cell arraged in order without inflammatory cell infiltrate .In irradiation of 4 weeks group , small amounts of inflammatory cell infiltrate , congesion and oedema were observed in sections .In irradiation of 12 weeks group , magnani-mous inflammatory cell infiltrate and significant congesion and oedema were observed in sections .Cells were necrosis , shedding , confused structure , and connective tissue in fibrosis .In irradiation of 16 weeks group , there were macrophage , inflammatory cell infiltrate and magnanimous fibrosis .With Azan staining, there was no fibrosis in control group , less blue fibrosis in irradiation of 4 weeks group, and magnanimous blue fibrosis in irradiation of 12 weeks group and irradiation of 16 weeks group .Proteins of TGF-βincreased with exposure time , and expression of NF-κB also increased with exposure time . Conclusion Irradiation could increase fibrosis and expression of TGF-βprotein,which might be related with the transcrip-tion of NF-κB.