Effect of osteopontin and CD44v6 in the progression of lung adenocarcinoma

WANG An-lei; SUN Bing-sheng; ZHANG Zhen-fa

doi:10.3969/j.issn.1002-266X.2014.33.001

Abstract：Objective To investigate the role of OPN and CD44v6 in the progression and invasion of lung adenocarci-noma.Methods OPN and CD44v6 expressions were detected in 129 patients with pericarcinomatous tissue , lung adeno-carcinoma in situ ( AIS ) and lepidic predominant adenocarcinoma ( LPA ) by immunohistochemical method .OPN and CD44v6 expressions were detected in H358 cells (lung adenocarcinoma in situ cell line ) and A549 cells by Western blot. Quantitative PCR was used to determine the expression levels of OPN mRNA and CD 44v6 mRNA.The influence of OPN and CD44 v6 on the invasive ability of H 358 cells and A549 cells was examined by Transwell chamber assay .Results Im-munohistochemical method revealed that the positive expression rates of OPN and CD 44v6 in LPA were significantly higher than those in AIS and pericarcinomatous tissue;the positive rates were higher in AIS compared to pericarcinomatous tissue . The expressions of these two markers were correlated positively (P<0.05).Western blot revealed that the positive expres-sion rates of OPN and CD44v6 in A549 cells were higher than those in H358 cells.Quantitative PCR revealed that the ex-pression levels of OPN and CD44v6 in A549 cells were higher than those in H358 cells.Transwell chamber assay revealed that OPN induced chemotaxis of cancer cells , and the chemotaxis was higher in A 549 cells than that in H358 cells.The mi-gration cell numbers of the two kinds of cancer cells decreased when blocked by OPN antibody .When the CD44 v6 receptor channel was blocked , OPN's chemotactic effect was weakened .Conclusion The OPN-CD44v6 complex plays an important role in lung adenocarcinoma progression;OPN might be used as an indicator to predict the progression and metastatic poten-tial of lung adenocarcinoma .