Effect of resveratrol on the autophagy of SGC7901 cells and its mechanism
CAI Ming-jie
PANG Xiao-yang
ZHU Dan
DONG Ying-yue
Abstract：Objective To study the influence of resveratrol ( RES) on the autophagy of SGC7901 cells and the role of classⅢPI3K.Methods SGC7901 cells were treated with different dose of RES (0, 10, 20, 50, 75, 100 μmol/L) for 24 h alone or pretreated with 3-MA ( or wortmannin, or chloroquine) for 1 h.Cell lysates were analyzed by Western blot for LC3-Ⅰ/LC3-Ⅱconversion;MDC staining was used to detect the morphologic changes of autophagy.Results The LC3-Ⅰ/LC3-Ⅱconversion and the number of autophagosomes increased in a dose dependent of RES and markedly increased in the presence of the protease inhibitors chloroquine.And the inhibitory function of class Ⅲ PI3K by its specific inhibitors ( wortmannin) had no impact on the level of autophagy induced by RES.Conclusion RES increased the autophagy of SGC7901 cells, and the autophagy was independent of classⅢPI3K.
Keywords：resveratrolstomach cancerautophagyclass ⅢPI3Kgastric cancer cell lines
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 20-22,112 )