Effect of paeoniflor in pretreatment to brain cells apoptosis and the expression of CHOP on cerebral ischemia reperfusion injury in rat
MAO Sen-lin
MA Yi-hong
ZHANG Hai-dong
QI Jun
LI Feng
Abstract：Objective To observe the influence of paeoniflorin ( PA) pretreatment to brain cells apoptosis and the ex-pression of CHOP on brain tissue of cerebral ischemia reperfusion injury ( CIR) in rat.Methods 72 male SD rats were randomly divided into 3 groups:pretreatment group, model group, sham operation group, each group of 24 rats.Pretreat-ment group was given PA 0.2 mg/kg by gavage, sham operation group and model group were given equal PBS by gavage once a day for 3 d.The fourth day after the treatment, CIR model were set up in pretreatment group and model group.6 rats in each group were taken out for detecting apoptosis index of brain tissue by TUNEL method, CHOP protein expression by immunohistochemical staining, at 1 h ( T0 ) , 4 h ( T1 ) , 24 h ( T2 ) after reperfusion.In each group, 6 rats were taken out for making neurological severity score at 24 h after reperfusion.Results At the same time point, the brain tissue cell apoptosis index and the CHOP protein expression level, model group>pretreatment group>control group, all P<0.05;the brain tissue apoptosis index and CHOP protein expression at T2 were higher than those in T0 , T1 in pretreatment group and model group, all P<0.05.The score of nerve function in pretreatment group and model group were higher than that in sham operation group, P<0.05 and 0.01 respectively;that in pretreatment group was lower than model group, P<0.01. Conclusion PA pretreatment can protect brain tissue of CIR rat, which maybe correlate with reducing apoptosis, CHOP protein expression and neural function score.
Keywords：cerebral ischemia reperfusionendoplasmic reticulum stressPaeoniflorinCHOP proteinapoptosis
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 14-16 )