Histological variation of the spleen cross-section after Radiofrequency during the partial splenectomy

CAO Lei; DING Bo; DONG Wei-gang; JIANG Tao; WANG Xi-mo; XU Jing

doi:10.3969/j.issn.1002-266X.2014.32.003

Abstract：Objective To observe the histological variation of the spleen cross-section after Radiofrequency (RF), and the influence of heat coagulation zone and its surrounding tissue after different RF time.Methods 12 pigs were ran-domly divided into observation group A, observation group B and the control group with 4 pigs in each group.Thermal co-agulation was established by multi point RF electrode in the midpoint of long axis perpendicular to the long axis of the spleen.RF time in Observation A and B groups were 2 and 5 min, the control group was not given energy.After wandering spleen, to observe the splenic tissue of coagulation band edge every 1 cm in group A and B, and the width of 3 mm of splenic tissue in control group, totally 5 tablets.Tissue morphology was observed with electron microscope and optical mi-croscope with HE staining;apoptosis was detected by TUNEL method, then apoptosis index was calculated.Results HE staining showed that the tissue necrosis of coagulation, cell structure totally destroyed;coagulation band around the splenic tissue no necrosis, no endovascular thrombosis formation, except the splenic sinus rupture.Related changes of RF thermo-coagulation hemostasis was observed in 2, 4 cm from coagulation band edge with transmission electron microscope in the ob-servation A and B groups.Reversible cell injury, hemorrhage, inflammatory exudation, and number of apoptotic cells in group A were lighter than those in group B.The apoptosis index in group B with 0, 1, 2 cm from coagulation, were higher than those in group A and control group, all P<0.01;apoptosis index in group A with 1, 2 cm from the coagulation, were higher than those in control group, all P<0.05.Conclusions The RF thermocoagulation is based on the spleen organiza-tion protein coagulation degeneration, blood vessels and sinusoidal thrombosis, vascular occlusion.The longer of RF time, the more serious of the damage surrounding spleen tissue.