Effect of ARF-BP1 gene on the apoptosis of HepG2 cells and its mechanism
QIN Xin-gan
LUO Dian-zhong
LV Zi-li
CHEN Gang
SU Chuan-li
Abstract：Objective To investigate the effect of ARF-BP1 gene on apoptosis of hepatoma HepG2 cells, and to ex-plore its mechanism.Methods HepG2 cells in logarithmic growth phase were randomly divided into 4 groups.Transfec-tion group was transfected translently by 100 nmol/L ARF-BP1 siRNA with LipofectamineTM 2000;liposome control group was only added liposomes; negative control group was transfected by negative siRNA fragments; blank control group was added the same amount of medium only, without siRNA fragments and liposomes.The flow cytometry was utilized to detect cells apoptosis rate of 4 groups; RT-PCR was used to detect the relative expression of p53, Mcl-1 mRNA in transfection group and blank control group.Results The apoptosis rate of transfection group, liposome control group, negative control group and blank control group were 27.90%±1.40%, 3.33%±0.66%, 3.05%±0.73% and 1.64%±0.12%, re-spectively;transfection group compared with other groups, all P<0.01; negative control group and liposome group com-pared with the control group, both P<0.05.72 h after transfection, p53, Mcl-1 mRNA relative expression in transfection group were 0.29 ±0.08 and 0.23 ±0.04, significantly lower than those in the blank control group ( P<0.01 and P<0.05).Conclusion Reduced ARF-BP1 gene expression in HepG2 cells can promote tumor cell apoptosis, which maybe correlate with inhibition of p53, Mcl-1 expression.
Keywords：hepatomaHepG2 cellubiquitinligating enzymeARF-BP1
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 4-6 )