Effects of interleukin 7 on different subsets of natural killer cells in multiple sclerosis patients
SU Ning
SHI Fu-dong
Abstract：Objective To study the effects of interleukin 7 ( IL-7) on different subsets of natural killer ( NK) cells in multiple sclerosis ( MS) patients.Methods The peripheral blood mononuclear cells from 10 MS patients were isolated and cultivated, then the subsets of NK cells and the expression of IL-7 receptors alpha (IL-7Rα) were measured.Mononuclear cells were stimulated with 50 ng/mL IL-7 or the same volume of culture media, the percentage of NK cells producing inter-feron gamma ( IFN-γ) and survival rate of NK cells by flow cytometry.Results Phenotype NK cells from MS patients by surface markers included of CD3-CD5+6 , CD3-CD1+6 and CD3-CD5+7 subsets, the mean fluorescence intensity ( MFI) of IL-7Rαexpression were 185.00 ±38.56, 139.30 ±14.30, 137.60 ±23.03, respectively;compared with CD-3 CD+56 NK sub-set, CD-3 CD+16 and CD-3 CD+57 NK subsets had lower IL-7Rαexpression (both P<0.05).Upon IL-7 stimulation, the per-centage of NK cells producing IFN-γincreased by 9.71%±2.59%, and the percentage of survival cells increased by 4.80%±1.88%on CD-3 CD+56 NK subset, which were both higher than those in CD-3 CD+16 and CD-3 CD+57 NK subsets, all P<0.01.Conclusion IL-7 stimulation promotes IFN-γproduction and survival rate of NK cells, and the effects are more prominent on CD3-CD5+6 subset than CD3-CD1+6 and CD3-CD5+7 subsets.
Keywords：multiple sclerosisinterleukin 7receptorinterferon gammasurvival
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1-3 )