Relationship between apolipoprotein B gene polymorphism and tendon-vessel stagnation syndrome of steroid induced osteonecrosis of the femoral head
ZENG Ping
LIANG Jing-chao
WEI Xiang-dong
WEI Biao-fang
Abstract：Objective To study the relationship between apolipoprotein B ( ApoB) gene polymorphism and the tendon-vessel stagnation of steroid induced osteonecrosis of the femoral head ( SONFH) .Methods A total of 108 patients with SON-FH were included in this study , including 64 patients with tendon-vessel stagnation syndrome of TCM ( tendon-vessel stagna-tion syndrome group ) and 44 patients with the deficiency of liver and kidney syndrome of TCM ( deficiency of liver and kidney syndrome group ) .There were 98 patients who adopt adrenal cortical hormone for treatment in 6 months without SONFH col-lected as control group .DNA of each group were extracted from the blood sample .The direct sequencing polymerase chain re-action and ligase detection reaction (PCR-LDR) technique were used to analyze XbaⅠ(rs693) and EcoRⅠ(rs1042031) single-nucleotide polymorphism of ApoB gene .Results Two gene type, X-X-and X+X-, were detected in XbaⅠsite.The amounts of X+X-genotype and X+allele frequencies of XbaⅠpolymorphism in tendon-vessel stagnation syndrome group were higher than those in deficiency of liver and kidney syndrome group (all P<0.05).Two gene type , which were E+E+and E+E-, were detected in EcoRⅠsite.The amounts of E+E-genotype and E-allele frequencies of EcoRⅠpolymorphism in tendon-vessel stagnation syndrome group were higher than those in deficiency of liver and kidney syndrome group (all P<0.05). However , the difference between the deficiency of liver and kidney syndrome and the control group showed no significance ( P>0.05).Conclusion The XbaⅠX+X-genotype, XbaⅠX+allele frequencies, EcoRⅠE+E-genotype and EcoRⅠE-al-lele frequencies may be related to the tendon-vessel stagnation syndrome of SONFH .
Keywords：steroid induced osteonecrosis of the femoral headapolipoprotein Bgene polymorphismtendon and ves-sel stagnation syndrome of TCM
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1-3 )