Comparison of detection results of the two methods on components and content determination of polysaccharides in low temperature extracts from Mongolian Terminalia chebula Ritz
LUO Xia
XUAN Cheng-rui
ZHAO Xiao-xia
ZHAO Peng-wei
SUN Peng
YANG Li-min
Abstract：Objective To estimate the detection results of the two methods on the determination of components and con -tent of polysaccharides in low temperature extracts from Mongolian Terminalia chebula Ritz .Methods The components and content of polysaccharides in five different positions were determined by infrared spectroscopy and anthrone -sulfuric acid color-imetric method.The infrared spectroscopy detection was completed in Tsinghua university analysis center .The precision, sta-bility, repeatability and average recovery rate of anthrone-sulfuric acid colorimetric method were evaluated by different experi-ments.Results The polysaccharides in the five different positions were determined by infrared spectroscopy , but no signifi-cant difference was found among the five different positions .In anthrone-sulfuric acid colorimetric method , the linear ranges of polysaccharides were 40-200 μg/mL, the precision was good ( RSD =0.460%) , the repeatability was good ( RSD =1.616%), and the average recovery rate was (110.130 ±0.010)%(RSD=0.946%).The reagent stability was good in 2 h at room temperature under natural light; linear regression equation of standard curve was Y =0.205 1X-0.003 4, R=0.999 1.There were polysaccharides in the five different positions , and the contents of polysaccharides were 14.15%-22.15%.Conclusions The component analysis of polysaccharides in the five different positions is preferably detected by in -frared spectroscopy , and anthrone-sulfuric acid colorimetric method is used to determine the contents of polysaccharides .
Keywords：Terminalia chebula Ritzpolysaccharidesinfrared spectroscopyanthrone-sulfuric acid colorimetry
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 27-29 )