Influence of exogenous nerve growth factor on expression of NF-κB mRNA of Schwann cells
XIN Lin-wei
WANG Li-ming
TANG Ji-cun
LI Zhao-xu
LI Qiang
Abstract：Objective To observe the influence of exogenous nerve growth factor ( NGF) on the expression of nuclear transcription factor-κB (NF-κB) mRNA of Schwann cells (SCs).Methods Schwann cells were isolated and cultured in vitro.The positive cells were counted by using S-100 immunohistochemical staining .The fourth generation of high-quality cells were randomly divided into the experimental group (NGF), blocking group (NGF+anti-NGFR) and control group (PBS).The total mRNA in each group were extracted and observed by ultraviolet spectrophotometer and 1.5%agarose gel electrophoresis , respectively , and the expression of NF-κB mRNA were detected by real-time fluorescence quantitative PCR.Results The living cell morphologies of SCs cultured in vitro were consistent with those observed under inverted mi -croscope.S-100 immunohistochemical staining showed that the purity of SCs was 91%.Ultraviolet spectrophotometer ob-served that A260/A280 was 1.8-2.2, 1.5% agarose gel electrophoresis showed that there were 28 and 18 s clear bands, which confirmed the quality of extracted mRNA was good .Real time fluorescence quantitative PCR in detection of expres-sion of NF-κB mRNA showed that when compared with the control group and blocking group , the expression of NF-κB mR-NA in the experimental group was significantly increased (all P<0.01).Conclusions Exogenous NGF can up-regulate the expression of NF-κB mRNA.
Keywords：nerve growth factorSchwann cellsnuclear transcription factor-κB
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 24-26 )