Protective effect of aliskiren on kidney of mice with adriamycin nephropathy

WU Qiao-yuan; LIAO Yun-hua; YANG Zhen-hua; GUO Yi; ZHOU Ying-chuan

doi:10.3969/j.issn.1002-266X.2014.29.008

Abstract：Objective To investigate the protective effect of aliskiren on kidney of mice with adriamycin nephropathy. Methods Twenty-four male BALB/c mice were subjected to the single tail vein injection of adriamycin to establish adriamycin nephropathy models.Then they were randomly divided into 4 groups, 6 in each group: the adriamycin nephropathy group, aliskiren group, valsartan group, and aliskiren +valsartan group;another 6 male BALB/c mice with the same age were selected as the control group.At week 2 and 6 after being injected adriamycin, the differences in urinary protein and kidney function were compared in each group.Mice were sacrificed at week 6.The paraffin-embedded sections of the kidney were stained by PAS, Masson and HE staining.The degrees of glomerular sclerosis and renal tubular damage were observed .Results Mice in the ad-riamycin nephropathy group showed proteinuria, hypoalbuminemia combined with progressive renal function decline, and ap-peared glomerulosclerosis and tubulointerstitial injury.Compared with the adriamycin nephropathy group, proteinuria improved and serum albumin increased significantly in the aliskiren group, valsartan group and aliskiren +valsartan group ( all P<0.05).Histological evaluation revealed that aliskiren group, valsartan group and aliskiren +valsartan group could significantly reduce the glomerulosclerosis and tubulointerstitial lesions (all P<0.05), but the aliskiren +valsartan group significantly im-proved the renal interstitial fibrosis (all P<0.05).Conclusion Aliskiren has protective effect on kidney in mice with adriamy-cin induced nephropathy and its combined application of valsartan can significantly improve the interstitial fibrosis .