Chemical compositions of Cortex Juglans Mandshurica and their activity screening of anti-hepatocarcinoma effect
CAO Zhi-jia
CAI Xiao-yan
FANG Wei
CHEN Hong-sheng
ZHANG Huai-nian
ZHANG Yong-li
Abstract：Objective To investigate the chemical compositions of Cortex Juglans Mandshurica and to evaluate anti-tumor effects of these compounds in human hepatoma cells.Methods Cortex Juglans Mandshurica were extracted with 75%ethanol, concentrated with chloroform and ethyl acetate, eluted radiantly on silica gel, and purified by preparative HPLC.Physicochemi-cal constants and spectroscopic analysis were used to identify their structures .MTT assay was used to evaluate their anti-hepato-carcinoma bioactivities.Results Two compounds were isolated and elucidated as luteolin and formononetin.Luteolin had strong cytotoxic effect on HepG2 and LO2 with IC50 values of (103.32 ±21.34) and (131.13 ±13.48) μmol/L;Formononetin showed lighter cytotoxic effect on HepG2 with IC50>200μmol/L.Conclusion Luteolin and formononetin are isolated from Cortex Jug-lans Mandshurica for the first time, and luteolin shows significant anti-tumor activity in human hepatoma cells.
Keywords：liver neoplasmsCortex Juglans Mandshuricachemical compositions
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1-3 )