Effect of forsythiaside on cisplatin-induced ototoxicity of guinea pigs
AN Ning
HOU Xue-dong
SHI Sheng-wu
Abstract：Objective To study the effect of forsythiaside on cisplatin-induced ototoxicity of guinea pigs .Methods Thirty guinea pigs were randomly divided into the control group (n=10), cisplatin group (n=10) and forsythiaside group (n=10).The guinea pigs in the cisplatin group were induced by intraperitoneal injection of cisplatin solution (8 mg/kg per day) for 7 days, the guinea pigs in the forsythiaside group were given forsythiaside (25 mg/kg per day) at 30 min be-fore cisplatin solution for 7 consecutive days , and the normal saline instead of cisplatin was injected in the control group . The distortion product otoacoustic emission ( DPOAE) was detected before the experimental animals were killed , and the expression of bcl-2 in cochlea of guinea pigs was detected by immunohistochemistry and Western blotting .Results Statis-tical difference was found in the DPOAE amplitudes under 1, 2, 4, 6 and 8 kHz test frequencies among the three groups:cisplatin group <forsythiaside group <control group (all P<0.05).Meanwhile, statistical difference was found in the expression of bcl-2 protein among the three groups:cisplatin group <forsythiaside group <control group (P<0.05 or P<0.01).Conclusion Forsythiaside can significantly reduce the cisplatin-induced ototoxicity by up-regulating the expres-sion of bcl-2.
Keywords：cisplatinforsythiasideototoxicitybcl-2guinea pig
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 21-23 )