Effect of ENO1 silencing on adhesion and invasion of human glioma U87 cells

GAO Yan; YANG Yu-yan; WANG Jing

doi:10.3969/j.issn.1002-266X.2014.28.006

Abstract：Objective To investigate the effect and mechanism of enolase-1 ( ENO1) silencing on the adhesion and invasion of human glioma U87 cells in vitro.Methods ENO1 silencing human glioma U87 cells were established , and then they were divided into the control group , no-load group, silenced group 1 and silenced group 2.The cell invasion and adhesion were detected by Transwell and MTS assay , and cell cycle was analyzed by flow cytometry .Real-time PCR and Western blot were used to detect the expression of CD 44, matrix metalloproteinase 2 (MMP-2), MMP-9, CDK1, p27, p53 and p-AKT.The levels of TGF-β, IL-1, IL-6 and IL-8 were measured simultaneously by multiplexed Luminex assay , and the transcription activities of NF-κB and Twist were detected by reporter gene assay .Results Compared with the control group, the invasion rate and adhesion abilities were decreased in the silenced groups 1 and 2 (all P<0.05), the cell cycle was arrested in G0/G1 phase, the protein and mRNA expression of p27 and p53 increased significantly ( all P<0.05), while the expression of CD44, MMP-2/9, CDK1 and p-AKT was down-regulated significantly (all P<0.05), the levels of TGF-β, IL-1βand IL-6 were decreased (all P<0.05), and the transcription activities of NF-κB and Twist were decreased (all P<0.05).Conclusion ENO1 silencing could inhibit the invasion and adhesion of human U 87 cells by regulating the related gene expression of cell cycle and invasion .