Effect of HS014 on OX-42 and TNF-αexpression in spinal cord tissues of morphine-tolerance rats
XU Hong-mei
NIU Ze-jun
CHU Hai-chen
Abstract：Objective To investigate of melanocortin receptor antagonist (HS014) on OX-42 and tumor necrosis fac-tor-α( TNF-α) expression in spinal cord tissues of morphine-tolerance rats .Methods Thirty adult male SD rats were ran-domly divided into 5 groups (n=6): morphine tolerance group (group M), normal saline group (group N), HS014+morphine group (group HM), normal saline +morphine group (group NM) and HS014+normal saline group (group HN) , each group was administered continuously for 5 d.All rats were sacrificed after the last dose on day 5 to obtain the L4-6 spinal cord tissues .The expression of TNF-αand OX-42 was examined by immunohistochemical technique .Results Compared with the group N , the expression of TNF-αand OX-42 was significantly increased in the groups M , HM and NM (all P<0.01).Compared with group M, the expression of TNF-αand OX-42 was significantly decreased in the group HM (all P<0.01).There was no significant differences between the other groups (all P>0.05).Conclusions Chronic use of morphine can induce microglial activation which shows that the expression of OX -42 and TNF-αis increased.HS014 can attenuate the morphine tolerance , whose mechanism may be related to the inhibition of morphine-induced activation of mi-croglia and TNF-αrelease.
Keywords：morphine tolerancemelanocortin receptormicrogliatumor necrosis factor-αOX-42rats
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 14-16 )