Effect of controlled low-pressure perfusion postconditioning on spinal cord ischemia-reperfusion injury in rabbits and the mechanism
AI Chun-yu
JIANG Xiao-jing
MA Hong
WANG Jun-ke
Abstract：Objective To investigate the effect of controlled low-pressure perfusion postconditioning on the spinal cord ischemia-reperfusion injury in rabbits and the mechanism .Methods Thirty-two Japanese white rabbits were randomly di-vided into four groups (n=8): the sham group (group S), ischemia-reperfusion (I/R) group, controlled low-pressure perfusion postconditioning group (group P), controlled low-pressure perfusion postconditioning +PD98059 (PD) group. All rabbits were sacrificed 24 h after reperfusion , L3-5 segments of the spinal cord were removed .TUNEL method was used to detect the apoptosis , the expression of p-ERK 1/2 and caspase-3 in the spinal cord tissues was detected by immunohisto-chemical SP method , the superoxide dismutase ( SOD) was determined by hydroxylamine method , and the malonyldialde-hyde ( MDA) in the spinal cord tissues was detected by thiobarbituric acid method .Results Compared with I/R group, the apoptosis index decreased, and in the spinal cord tissues, MDA decreased, SOD increased, caspase-3 decreased, p-ERK1/2 increased in the group P (all P<0.05);compared with group P, the apoptosis index increased , and in the spinal cord tissues, MDA increased, SOD decreased, caspase-3 increased, p-ERK1/2 decreased in the PD group ( all P <0.05).Conclusions Controlled low-pressure perfusion postconditioning has the protective effect on the spinal cord I /R injury, whose mechanism may be related to activation of ERK 1/2 pathway and inhibition of the expression of caspase-3.
Keywords：ischemia-reperfusion injurycontrolled low-pressure perfusion postconditioningspinal cordJapanese white rabbits
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 7-9 )