Inhibition effect and mechanism of harmine on proliferation of human leukemia HL-60 cells

SUN Ya-jun; ZHANG Chun-de

doi:10.3969/j.issn.1002-266X.2014.27.008

Abstract：Objective To investigate the inhibition effect and mechanism of harmine on proliferation of human leuke -mia HL-60 cells.Methods Human leukemia HL-60 cells were treated with different concentrations of harmine .The effect of harmine on proliferation of HL-60 cells was observed by MTS assay , and cell cycle was analyzed by flow cytometry . Western blotting and RT-PCR were used to detect the expression changes of STGC 3, CDK1, p27, p53 and p-AKT protein and mRNA, the levels of TGF-β, IL-1, IL-6 and IL-8 were measured simultaneously by ELISA assay , and transcription ac-tivities of NF-κB and AP-1 were detected by reporter gene assay .Results The proliferation of HL-60 cells was inhibited by harmine and in a dose-dependent manner .HL-60 cells were dealed with 1 μg/mL and 2 μg/mL harmine, the cells in G0/G1 phase increased and in S/G2/M phase decreased; the protein expression of CDK 1 and p-AKT was down-regulated significantly, but the protein expression of STGC3, p27 and p53 was up-regulated significantly; the mRNA expression of CDK1 was down-regulated, but the mRNA expression of STGC3, p27 and p53 was up-regulated;and the transcription ac-tivity of NF-κB and AP-1 was decreased , the production of cytokines was decreased .Conclusion Harmine could inhibit the proliferation of HL-60 cells by down-regulating CDK1, down-regulating STGC3, p27 and p53, and inhibiting the levels of TGF-β, IL-1, IL-6 and IL-8 and the transcription activities of NF-κB and AP-1.