Inhibitory effect of baicalein on proliferation of cervical carcinoma Hela cells and its mechanism
LU Ke-lian
YU Xiao-lan
XIA Ji-yi
MAO Xi-guang
Abstract：Objective To observe the inhibitory effect of baicalein on the proliferation of cervical carcinoma Hela cells and to investigate its possible mechanism .Methods Hela cells cultured in vitro were randomly divided into 3 groups:group A which was added 100μmol/L baicalein, group B which was added 200μmol/L baicalein and group C which was added se-rum-free DMEM high-glucose medium without baicalein , and they were all treated for 24 hours.The morphology and quantity of Hela cells were observed under phase-contrast microscope , the cell cycle of each group was detected by flow cytometry , the activities of matrix metalloproteinases ( MMP-2 and MMP-9 ) were detected by gelatin zymography , the mRNA expression changes of MMP-2, MMP-9, murine sarcoma protein (Ras), Cyclin D1 and integrinβ1 were measured by RT-PCR, and pro-tein expression changes of MMP-2, MMP-9, Ras, Cyclin D1 and integrinβ1 were measured by Western blotting .Results Compared with group C , the cell quantities of groups A and B were decreased , and the cell morphology changed from irregular similar circular to multi-angle spindle;the cell percentages in the S phase of groups A , B and C were 24.58%, 19.41%and 34.05%;and the cell percentages in the G 1 phase were 72.67%, 79.17%and 64.88%, respectively;and statistical differ-ence was found between groups A , B and C (all P<0.05);the activities of MMP-2 in groups A, B and C were 32.651 ± 0.571, 28.987 ±1.033 and 36.127 ±1.184;the activities of MMP-9 in groups A, B and C were 33.217 ±0.660, 29.277 ± 0.868 and 39.613 ±0.318;and statistical difference was found between groups A , B and C ( all P<0.05);the mRNA ex-pression of MMP-2, MMP-9, Ras, Cyclin D1 and integrin β1 in the group A was 1.012 ±0.045, 1.009 ±0.030, 0.988 ± 0.036, 1.013 ±0.034 and 0.983 ±0.036;it was 0.849 ±0.026, 0.914 ±0.027, 0.626 ±0.029, 0.806 ±0.037 and 0.799 ±0.037 in the group B;it was 1.199 ±0.029, 1.087 ±0.024, 1.413 ±0.026, 1.203 ±0.029 and 1.224 ±0.025 in the group C, and statistical difference was found between groups A , B and C (all P<0.05);the protein expression of MMP-2, MMP-9, Ras, Cyclin D1 and integrinβ1 in the group A was 0.693 ±0.025, 0.886 ±0.032, 1.127 ±0.028, 1.061 ±0.028 and 1.159 ±0.031, in the group B was 0.326 ±0.022, 0.754 ±0.024, 0.782 ±0.183, 0.321 ±0.027 and 0.481 ±0.028, and in the group C was 1.914 ±0031, 1.487 ±0.016, 1.323 ±0.303, 1.816 ±0.025 and 1.467 ±0.036;and statistical difference was found between groups A , B and C (all P<0.05).Conclusion Baicalein inhibits the proliferation of cervical cancer Hela cells by arresting the cell in the G 1 phase, decreasing the activities of MMP-2 and MMP-9, and down-regulating the mRNA and protein expression of MMP-2, MMP-9, Ras, Cyclin D1 and integrin β1.
Keywords：baicaleincervical carcinomacervical neoplasmsHela cellsmatrix metalloproteinasesmurine sarcoma proteinCyclin D1integrin β1
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 4-7 )