The study of long-term brain protective effect and mechanism of isoflurane postconditioning on hypoxic-ischemic brain injury in neonatal rats
JI Guo-yu
XUE Hang
YU Wei-wei
JI Hai-yin
YANG Ya-ting
JI Ping
Abstract：Objective To investigate long-term brain protective effect and mechanism of isoflurane postconditioning on hypoxic-ischemic brain injury(HIBI) in neonatal rats.Methods 105 Sprague-Dawley(SD)neonatal rats were randomly divided into sham surgery group , model group , isoflurane group , atractyloside group , atractyloside with isoflurane group , cyclosporine group and cyclosporine with isoflurane group .Each group had left common carotid arterial ligation ( sham group threading without ligation ) followed by 8% oxygen and 92% nitrogen mixed gas .After model was established ( or sham surgery), injected drug in lateral ventricle: two atractyloside group were injected atractyloside , two cyclosporine group were injected cyclosporine A , other groups were injected saline .1.5%isoflurane were inhaled in isoflurane process-ing group for 30 min.Evaluating cognitive function by Morris water maze test (MWM) in the 30th to 35th days.Neuronal density ration in thalamic ventral posteromedial nucleus and hippocampal CA 3 region of left to right cerebral hemisphere were calculated after the MWM .Results Compared with sham surgery group , the average escape latency time and swim-ming routes was longer(P<0.05), while the number of crossing platform, the percentage of platform quadrant time and platform quadrant routes were reduced in model group , two atractyloside group , and there was no significant difference in isoflucane group and two cyclosporine group , while neuronal density ration were reduced(P<0.05)in other groups.Com-pared with model group, the average escape latency time and swimming routes were shorter (P<0.05), while the number of crossing platform , the percentage of platform quadrant time and platform quadrant routes and neuronal density ration were increased(P<0.05)in isoflurane group and two cyclosporine group .While there was no significant difference in above in-dex in two atractyloside group(P>0.05).Compared with isoflurane group, the average escape latency time and swimming routes were longer ( P<0 .05 ) , while the number of crossing platform , the percentage of platform quadrant time platform quadrant routes and neuronal density ration were reduced (P<0.05) in two atractyloside group(P<0.05),and there was no significant difference in two cyclosporine group (P>0.05).Conclusion Isoflurane postconditioning has long-term pro-tective effect on reducing HIBI neonatal rats , and the mechanism may be by inhibiting the openness of mPTP .
Keywords：IsofluranepostconditioningHIBIMWMLearningMemory
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )