Effects of Gekko sulfated glycopeptides on proliferation of human breast cancer SKBR3 cells and the mechanism
WANG Ya-nan
WU Xiong-zhi
Abstract：Objective To observe the effects of Gekko sulfated glycopeptides ( GSPP ) on the proliferation of human breast cancer SKBR3 cells and to explore its mechanism .Methods Human breast cancer SKBR3 cells were cultured in vitro.GSPP 100 μg/mL, basic fibroblast growth factor ( bFGF) 5 ng/mL, GSPP 100 μg/mL and bFGF 5 ng/mL were added to SKBR3 cells, respectively.Moreover, PBS negative control group was designed in the study .After 72 h, cell pro-liferation was detected by trypan blue staining method;cell cycle was tested by flow cytometry;and the secretion of cyclin D1 was tested by ELISA .Results Compared with the negative control group , GSPP had inhibitory effect on cell prolifera-tion of SKBR3 cells (P<0.01), and bFGF had promoting effect on cell proliferation (P<0.01).The number of cells in the combination group had no difference between the combination group treated by GSPP and bFGF and the PBS negative control group.The GSPP arrested cells in the G1 phase and decreased the secretion of cyclin D1 (all P<0.05).The bFGF decreased the proportion of cells in G 0/G1 phase and increased the secretion of cyclin D 1 (all P<0.05).The proportion of cells in the G0/G1 phase and secretion of cyclin D1 in the combination group had no statistical difference as compared with that of the PBS negative control group .Conclusions GSPP can inhibit SKBR3 cells proliferation by decreasing the level of cyclin D1.What's more, GSPP acts as an antagonist for bFGF and inhibits the promoting effect of bFGF on cell prolifera-tion.
Keywords：breast neoplasmsGekko sulfated glycopeptidesbasic fibroblast growth factorcell proliferationcyclin
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1-3 )