Expression changes and significance of STAT3 and cyclin D1 in hepatocellular carcinoma tissues of rats
Abstract：Objective To observe the expression changes of signal transducer and activator of transcription 3 (STAT3) and cyclin D1 in hepatocellular carcinoma tissues of rats and to investigate its significance .Methods Seventy-two rats were randomly divided into the model group and control group , 36 rats in each group .The model group received 0.1 mg/mL diethylnitrosamine (DEN) freely, and the control group was given the same volume of sterile distilled water for 20 weeks.The STAT3 and cyclin D1 mRNA was detected by using RT-PCR, Western blotting and immunohistochemistry were used to detect the STAT 3 and cyclin D1 protein expression .Results In control group , the mRNA expression of STAT3 and cyclin D1 were respectively 0.32 ±0.12 and 0.18 ±0.05, in the model group respectively 0.72 ±0.25 and 0.57 ±0.15, all P<0.05;in the control group, the protein expression levels of STAT3 and cyclin D1 were respectively 0.32 ±0.19 and 0.41 ±0.15, while in the model group , they were respectively 0.58 ±0.25 and 0.65 ±0.14, all P<0.05;immunohistochemical results showed that the IOD of STAT 3 and cyclin D1 in the model group was respectively 2 373.87 ±258.79 and 668.44 ±63.10, in control group, it was respectively 487.81 ±55.31 and 138.86 ±31.50, all P<0.05.Conclusion The expression of STAT3 and cyclin D1 in the hepatocellular carcinoma tissues of rats is up-regula-ted, and both of them can promote the occurrence and development of hepatocellular carcinoma .
Keywords：liver neoplasmsprimary hepatocellular carcinomadiethylnitrosaminesignal transducer and activator of transcription 3cyclin D1rats
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 16-18 )