Effects of high concentrations of uric acid on oxidative stress of human renal tubular epithelial cells
ZHANG Tao
NIU Hong-lin
KANG Ying-li
LI Ying
Abstract：Objective To observe the effects of high concentrations of uric acid ( UA) on oxidative stress of human proximal renal tubular epithelial cells , and to investigate the possible pathway .Methods HK-2 cells were cultured in vitro and exposed to 720μmol/L UA for 0 h, 12 h, 24 h and 48 h.The reactive oxygen species ( ROS) production in cells was detected by DCHF-DA staining.The MDA content and T-SOD activity in the supernatants were measured by Spectro-photometer .The mitochondrial ROS production was detected by MitoSOXTM staining .Results There were no significant changes in ROS production, MDA content and T-SOD activity of HK-2 cells exposed to UA for 12 h (all P>0.05).The cell ROS production and MDA content increased , while T-SOD activity decreased when HK-2 cells were exposed to UA for 24 h.Changes were more significant at 48 h (all P<0.05).The mitochondrial ROS production was increased in HK-2 cells exposed to UA for 24 h, and it increased more when exposed to UA for 48 h (P<0.05).Conclusion High concen-trations?of UA can induce the oxidative stress in HK-2 cells through the mechanism of increasing the mitochondrial ROS production .
Keywords：Metabolic Diseasesrenal tubular epithelial cellsuric acidmitochondriareactive oxygen speciesoxi-dative stress
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 13-15 )