Relationship between initial platelet shape change and aggregation and analysis of influential factors
MAO Ya-li
HAN Ji-ju
WANG Yun
CHANG Huan
XIA Zuo-li
ZHAO Xiao-min
Abstract：Objective To investigate the relationship between initial platelet shape change and aggregation and to ana -lyze the influential factors .Methods After the platelet rich plasma ( PRP) in mice and rats and human washed platelets were prepared, platelet count was adjusted with normal saline (NS), platelet poor plasma (PPP) or Tyrode′s buffer.Neg-ative peak ( NP) and time to reach negative peak ( TNP) and maximum aggregation induced by adenosine monophosphate ( ADP) or collagen were determined by the turbidimetric method .Results Human NP and TNP were more than that of mice (all P<0.05), only human NP was more than that of rats (P<0.01), whereas rat TNP was more than that of mice (P<0.01).Human TP decreased and TNP prolonged significantly (all P<0.05) in PRP diluted with NS as compared with that diluted with PPP.Compared with ADP, collagen decreased NP (P<0.05) and increased TNP (P<0.01) in human PRP, and increased NP and TNP (P<0.01) in human washed platelets.Both ADP and collagen decreased NP and TNP in washed platelets than in PRP with the exception of increase in NP induced by collagen (all P<0.01).Multivariate regression analysis showed that aggregation was positively correlated with NP (r=0.49, P<0.01) and TNP (r=0.48, P<0.01).Conclusions Initial platelet shape change enhances aggregation .It is more in human than in rats and mice, is less in PRP diluted with NS and washed platelets , decreases in PRP with collagen and increases in washed platelets with ADP.
Keywords：blood plateletsaggregationplatelet systemagonistplatelet shape changeoptical methodmicerats
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1-3 )