Construction of eukaryotic expression vector expressing truncated NDRG2
Abstract：Objective To construct pCNDA4.0-NDRG2 vectors that correctly express the truncated N-MYC down-stream-regulated gene 2 (NDRG2).Methods The truncated NDRG21-178aa and NDRG21-257aa of NDRG2 was amplified by PCR, and were digested by BamHⅠand EcoRⅠ, then were cloned into the expression vector pCNDA 4.0 by SolutionⅠlig-ase reaction system.The protein expression of vectors was detected by Western blotting .Results The truncated NDRG2 was correctly expressed in the pCDNA 4.0 expression vector .Conclusion The eukaryotic expression vector of truncated NDRG2 is successfully constructed .
Keywords：N-MYC downstream-regulated gene 2truncated mutantspCNDA4.0 vector
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 9-12 )