Spontaneous chloride currents in poorly differentiated nasopharyngeal carcinoma cells
YANG Xiao-ya
XIE Wen-rui
ZHU Lin-yan
MAO Jian-wen
WANG Li-wei
CHEN Li-xin
Abstract：Objective To explore the spontaneous chloride currents in poorly differentiated nasopharyngeal carcinoma ( CNE-2Z ) cells.Methods The whole-cell patch-clamp technique was used to detect the Cl -currents.The characteris-tics of the currents were clarified by ATP depletion in the pipette solution , extracellular applications of chloride channel blockers and hypertonic challenges .Results A chloride current was activated spontaneously and occasionally in the iso-tonic solution in CNE-2Z cells, showing significant outward rectification but no time-dependent inactivation .The reversal potential of the current was (-7.6 ±0.9) mV, which was close to the calculated equilibrium potential for Cl -.The in-duction of the chloride channel was dependent on the intracellular ATP .The spontaneous chloride currents were inhibited by extracelluar applications of the chloride channel blocker ATP and 47% hypertonic solution .Conclusion A chloride channel can be activated spontaneously and occasionally under the isotonic condition in CNE -2Z cells, showing the similar characteristics to the volume-sensitive chloride channel .
Keywords：nasopharyngeal neoplasmschloride channelspatch-clamp techniqueCNE-2Z cells
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 5-8 )