Effect of IGF-1R silencing mediated by RNAi on EMT in non-small-cell lung cancer PC-9/ZD cell line
ZHANG Xi
ZHANG Wei-min
CHEN Bei
Abstract：Objective To investigate the effect of insulin-like growth factor 1 receptor ( IGF-1R) gene silencing medi-ated by RNA interference ( RNAi) on cell epithelial-mesenchymal transition ( EMT) in non-small-cell lung cancer PC-9/ZD cells.Methods The recombinant lentivirus specific to IGF-1R gene was constructed to be transfected into EGFR-TKIs-resistant non-small-cell lung cancer PC-9/ZD cells.Real-time quantitative RT-PCR was used to detect the inhibition rate of IGF-1R in PC-9/ZD cells;meanwhile, after IGF-1R was inhibited, the mRNA expression of E-cadherin and Vimen-tin was detected , and the invasive and migratory capabilities of cells were determined by Transwell assay .Results The RNAi recombinant lentivirus targeting IGF-1R was successfully constructed .The final titer acquired was 1 ×106 TU/μL, and the inhibition rate of IGF-1R mRNA was 71.4%.After the IGF-1R gene was inhibited, the PC-9/ZD cells displayed epithelial phenotypes, and their capabilities of invasion and migration were significantly decreased (P<0.05).The ex-pression of epithelial cell marker E-cadherin mRNA was increased (P<0.05), accompanied by down-regulation of Vimen-tin mRNA level (P<0.05).Conclusion IGF-1R silencing can reverse cell EMT in non-small-cell lung cancer PC-9/ZD cells.
Keywords：RNA interferencegene silencinginsulin-like growth factor 1 receptorepithelial-mesenchymal transi-tionnon-small-lung cancerepidermal growth factor receptor-tyrosine kinase inhibitorsacquired drug resistance
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1-4,110 )