Effect of hesperidin on remineralization process of bovine root dentine in vitro

YU Cui-ping; LI Mei-jia; ZHANG Xiang-yu; YANG Wen-hua

doi:10.3969/j.issn.1002-266X.2014.22.005

Abstract：Objective To investigate the effect of hesperidin on the remineralization process of bovine root dentine in vitro.Methods Twenty-six extracted young permanent bovine teeth were prepared into 6 mm ×6 mm ×2 mm size of den-tin slices, and randomly divided into3 groups:the research group, positive control group and blank control group , six slices in each group.Specimens were immersed in demineralizing solution for 96 hours, and then we formed dentin demineraliza-tion lesions.Specimens were incubated in testing solutions (5 000 mg/L hesperidin, 1 000 mg/L NaF and deionized wa-ter) for 2 hours and remineralizing solution for 8 hours, for 8 days.The remineralizing solution and dentin slices of each group were collected .Hydroxyproline content of the remineralizing solution was investigated by hydroxyproline assay , den-tine Ca and P contents were analyzed by energy-dispersive X-ray spectrometry .Results Hydroxyproline contents of the research group, positive control group and blank control group in the remineralizing solution were respectively (22.80 ± 11.78), (16.43 ±7.87) and (64.16 ±3.92).Hydroxyproline contents of the research group and the positive control group were higher than that of the blank control group (P<0.05).Dentine Ca/P ratios of the research group and the posi-tive control group were higher than that of the blank control group , the research group was lower than the positive control group (all P<0.05).Conclusion Hesperidin inhibits dentine collagen degradation and enhanced remineralization in vitro.