Influence of ML-7 intragastric administration on contents of trace elements in rabbits′kidney with atherosclerosis
WU Yun-kai
WANG Hu
WEI Wen-mei
GU Zhi-hong
WANG Yuan
Abstract：Objective To explore the influence of myosin light chain kinase ( MLCK) inhibitor ML-7 on the contents of Fe, Mn, Cu, Zn, Se, Pb and Cd in rabbits'kidney with atherosclerosis ( AS) .Methods Forty rabbits were randomly divided into 3 groups:ML-7 treatment group (n=17), high-fat diet group (n=13) and normal control group (n=10). Rats in the ML-7 treatment group were fed with high-fat food, from the 6th week on, 5 mg/(kg· d) ML-7 was given via intragastric administration for 12 weeks.High-fat feeding was given to rats in the high-fat diet group for 12 weeks.Rats in the normal control group were given basal feeding for 12 weeks.After feeding for 12 weeks, blood was collected from the carotid of the rabbits in each group after anesthesia .Then kidney tissue were taken immediately after the killing of the rab-bits.TC level in the carotid blood was detected , and the contents of Fe , Mn, Cu, Zn, Se, Pb and Cd in the kidney of each group was analyzed by using ICP-AES.Results TC levels in the ML-7 treatment group , high-fat diet group and nor-mal control group were (21.68 ±4.69), (28.33 ±1.95) and (1.03 ±0.70) mmol/L respectively.TC levels in the ML-7 treatment group and high-fat diet group were higher than that of the normal control group , while TC level in the ML-7 treatment group was lower than that of the high fat diet group (all P<0.05).Compared with the normal control group , the contents of Zn, Mn and Se decreased, while Fe content increased (all P<0.05).Compared with the high-fat diet group, the contents of Zn, Mn and Se increased, while Fe content decreased (all P<0.05).Conclusions ML-7 can decrease TC level in the serum of model rabbits , increase Zn, Mn and Se contents in rabbits'kidney with AS.Meanwhile, ML-7 plays a role of resistance to the AS by increasing the Zn , Mn and Se contents in the kidney .
Keywords：myosin light chain kinaseatherosclerosiskidneytrace elements
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 5-7 )