Inhibiting RhoA expression to induce apoptosis of breast cancer cells
Abstract：Objective To investigate the role of RhoA gene inhibition in inducing the apoptosis of breast cancer (BC) cells.Methods Based on a previously constructed adenoviral vector-mediated siRNA system, we infected the BC MCF-7 cells.After cell culture and virus titer measurement experiment , we collected the virus sample .Then Western blot -ting and RT-PCR were used to detect the expression levels of protein and mRNA , respectively .MCF-7 cells were infected by Adsi RNA-RhoA and detected by using MTT and DNA fragmentation assays .Results The Adsi RNA-RhoA infected BC cells significantly inhibited the expression of RhoA gene ( the inhibition rate 75.64%) and reduced the transcriptional level of RhoA mRNA (69.44%).MTT assay revealed that the growth and proliferation of MCF-7 cells infected by Adsi RNA-RhoA was inhibited .TUNEL assay indicated that Adsi RNA-RhoA could induce the apoptosis of MCF-7 cells.Con-clusion Using siRNA to inhibit the expression of RhoA gene in BC cells can induce the apoptosis of BC cells .
Keywords：breast carcinomaRhoA genesmall interfering RNAapoptosis
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 4-6 )