Potential mechanism of carcinogenesis and cytotoxicity of diesel exhaust particles
ZHOU Fang
LI Su-li
JIA Wen-liang
KANG Chun-sheng
ZHANG Qing-yu
Abstract：Objective To investigate the potential mechanism of carcinogenesis and cytotoxicity of diesel exhaust par -ticles (DEP) by observing the changes of epidermal growth factor receptor (EGFR), PI3K and AKT when we added DEP into the normal human bronchial epithelial ( HBE) cells.Methods DEP of 0, 50, 100, 200 and 400 μg/ml was added respectively into the studied HBE cells , and the expression change of EGFR was detected by Western blotting and then we chose the most obvious stimulating concentration as the research object .The expression changes of EGFR , PI3K, AKT and p-AKT were detected by Western blotting .The toxicity changes of HBE cells were observed , the cell invasion ability was detected by Transwell , and the cell cycle and apoptosis by flow cytometry .Results The expression of EGFR in HBE cells when treated with 100 μg/ml DEP was significantly higher than that in other concentrations (P<0.05).Western blotting showed that the expression of PI3K, AKT and p-AKT in HBE cells of the treatment group was higher as compared with that of the control group .The cytotoxicity results also showed that , after treating with DEP , the cell cycle of HBE cells was ar-rested in G0/G1 phase, the apoptosis rate was increased and the invasive ability was decreased .Conclusion DEP have specific cytotoxicity , and the increased expression of EGFR may play an important role in the process of lung carcinogenesis coursed by DEP, whose potential mechanism may be related to activation of PI 3K/AKT pathway.
Keywords：diesel exhaust particlesnormal human bronchial epithelial cellsepidermal growth factor receptorcytotoxicity
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 5-8 )