Induction of preeclampsia rat model by injection of low-dose endotoxin

LI Jing; TANG Hui

doi:10.3969/j.issn.1002-266X.2014.19.008

Abstract：Objective To induce the rats to copy the rat model with preeclampsia by using low-dose lipopolysacchar-ides.Methods:twelve pregnant rats were randomly divided into 2 groups: experimental group and normal control group , with 6 rats in each group.The experimental group were injected with 2ml of lipopolysaccharides solution of 1μg/kg through tail veins on day 14 of pregnancy, while the control group were injected with 2ml saline.The blood pressure on day 7,13, 15,17,19 were monitored, and the 24-hour proteinuria on day 12,15 were measured by Immune turbidimetry .All the rats had no delivery on day 20, and then the uterine cesarean delivery were made to them , and observed the status of the new-born mouse, and got the placentas to make HE staining and observed the pathological changes .Results:The systolic pres-sure of the experimental group rose significantly on day 15,17 and 19 of pregnancy, which was higher more than 30 mmHg of the basic pressure .The blood pressure of the normal control group had no obvious change .The P of both two groups were less than 0.05 while they compared with each other .On day 15 of pregnancy, the 24-hour proteinuria(more than twice as much as the basic pressure) of the experimental group was(6.07 ±0.75)mg/24h, while that of the normal group was (1. 90 ±0.43)mg/24h, and P<0.05 when these 2 groups compared with each other .The experimental group showed placen-tal inflammatory changes such as baseband inflammatory cell infiltration , labyrinth zone tissue nuclear fragmentation and fi-brin deposition by examination of the pathology .Conclusion:low-dose lipopolysaccharides could induce preeclampsia per-formances such as hypertension , proteinuria and placental inflammatory changes in rats .The The rat model with preeclamp-sia was successfully established .